CV of Hector Quemada
My Bio
I am a retired biologist, with a Ph.D. in Plant Molecular Biology. My last position was as a Principal Research Associate at Western Michigan University, working with the Foundation for the National Institutes of Health on a project aimed at strengthening capacity of regulators in Africa, for regulating gene drive technologies. Prior to this, I was Director of the Biosafety Resource Network at the Donald Danforth Plant Science Center, a project that provided regulatory and product development expertise for publicly funded transgenic crop development projects. Before joining the Danforth Center, I was the manager of the Biotechnology and Biodiversity Interface grant component of the Program for Biosafety Systems, a USAID project supporting research to generate data relevant to risk assessments of crops in developing countries. I was the founder of Crop Technology Consulting, Inc., a consulting firm conducting technical and biosafety assessment for biotechnology programs in developing countries, and developing regulatory approval dossiers for public and private crop development organizations. I have had experience developing transgenic crop varieties for the private sector.
My Work with the Foundation for the National Institutes of Health (GeneConvene)
To date, applications of genetic engineering have been in agricultural or industrial settings, with domesticated populations of organisms. Gene drives are a new development in the field of genetic engineering, which provides opportunities to alter the genetic composition of natural populations. This ability will allow significant benefits to public health, through the control or modification of disease vectors, such as mosquitoes. It will also provide a novel means of controlling pests and invasive species, thus preserving agricultural productivity and biodiversity. My project at Western Michigan University was to support efforts of the Foundation for the National Institutes of Health, in partnership with the African Union Development Agency, to strengthen the capacity of regulators in African countries to handle future applications of gene drive technology. These organisms fall under most countries’ laws governing genetically engineered organisms, but the application of these laws and development of regulations will require a good understanding of the technology, its potential, and its risks.
Name
Hector D. Quemada
Education and Degrees
1979-1986 University of Utah, Salt Lake City, Utah.: Ph.D., Biology (Cell and Molecular Biology).
1977-1979 University of Kansas, Lawrence, Kansas.: M. A., Botany.
1973-1977 University of Kansas, Lawrence, Kansas.: B.S., Biology (Systematics and Ecology) with honors, summa cum laude.
Employment History
July 2022-Present Retired
December 2018-June 2022 Principal Research Associate, Department of Biological Sciences, Western Michigan University
Led a group of consultants to work with the Foundation for the National Institutes of Health and the African Union Development Agency to provide regulators in several countries in Africa with the necessary technical background and strengthened capacity to handle applications for research and eventual deployment of gene drive technology.H
November 2008-December 2018 Director, Biosafety Resource Network, Donald Danforth Plant Science Center
Direct a worldwide network of consultants to provide regulatory science support and capacity building for public sector genetic engineering projects. Projects involve development of regulatory approval dossiers, regulatory strategy, regulatory agency consultations, assisting regulatory capacity building organizations, participation in international regulatory policy development. Current projects include regulatory capacity building and policy support for gene drive research in Africa; development of regulatory approval packages for transgenic crops, including virus resistant and biofortified cassava and insect resistant cowpea. Extensive experience working in Africa and Asia.
2008-2010 Adjunct Professor of Biology, Calvin College
Team-teach Biology and Biotechnology courses; organize and implement interim session classes.
2003-2010 Manager, Biotechnology and Biodiversity Interface grant component, Program for Biosafety Systems
Manage BBI grant program, funding research conducted by African and Asian to assess the impact of genetically engineered crops on the environment. Provide technical support to developing countries in the area of biosafety regulations and policy for USAID’s Program for Biosafety Systems.
1997-2008 President and Principal Consultant, Crop Technology Consulting, Inc.
Prepared submissions to US and foreign regulatory agencies for movement, field trials, and product approval of transgenic crops; coordinate and conduct contract research for client companies to generate environmental risk assessment and food/feed safety data required for regulatory approvals of products; manage research collaborations and product development. Clients include small and regional-based agricultural technology companies, and a major land grant university.
Advised foreign government clients and USAID in the area of agricultural biotechnology policy, research capability, and biosafety. Foreign assignments include consultations in India, the Philippines, Egypt, Uganda, Kenya, South Africa, Indonesia and Thailand.
2002-2005 Executive Vice President and Treasurer, Avenir Associates.
Provided interim management services to clients in the life sciences industry. Assisted clients in developing and implementing regulatory compliance programs.
2007-2008 Adjunct Associate Professor of Biology, Calvin University
2003-2007 Principal Research Associate, Western Michigan University
2000-2003 Adjunct Professor of Biology, Western Michigan University
1995-2000 Adjunct Assistant Professor of Biology, Western Michigan University
Conducted research activities funded by USDA: the study of long-term ecological consequences of release of transgenic crops, using squash as a model system. Lecturing in food science and plant biology courses.
1996-1997 Associate Director, Biotechnology, Asgrow Seed Company
Responsible for establishing an internal biotechnology research group for corn, soybeans, sunflower, and sorghum. Participate in corporate strategic planning. Responsible for developing and implementing strategic plan for biotechnology research program, technology acquisition, management of external collaborations and contracts, regulatory affairs, licensing, patent issues.
1994-1996 Associate Director, Vegetable Biotechnology and Seed Technology, Asgrow Seed Company
Managed a biotechnology research group; managed a seed biology research group. Responsible for strategic planning for the biotechnology program, managing external collaborations, project feasibility assessment, product development, regulatory affairs, patents, and licensing.
1991-1994 Associate Director, Vegetable Biotechnology, Asgrow Seed Company.
Managed a biotechnology research group. Responsible for managing external collaborations, project feasibility assessment, product development, regulatory affairs, patents, and licensing.
1989-1991 Vegetable Project Manager, Asgrow Seed Company.
Managed a research group and participated in biotechnology research; participated in field research with transgenic plants. Also responsible for regulatory affairs, patents, and licensing.
1986-1989 Visiting Scientist, The Upjohn Company.
Cloned and engineered viral genes to confer viral resistance to plants; introduced these genes into plants as proof of concept.
Funded Research
Gene Flow from Transgenic Cucurbita pepo into “Free-living” populations. USDA Risk Assessment Grant, 1999-2002. $130,000.
Other Funded projects
Biotechnology/Biodiversity Interface grant program, component of USAID Program for Biosafety Systems. 2003-2008. $7,605,039.
Regulatory Development for HEG-modified mosquitoes. Foundation for the National Institutes of Health. 2012-2016. $1,096,600.
Regulatory Capacity Building for Gene Drive Research. Foundation for the National Institutes of Health. 2016-2019. $1,217,626.
Transitional Support for Gene Drive Research. Foundation for the National Institutes of Health. 2017-2019. $2,110,008.
Support for FNIH Gene Drive Research Coordination. 2019-2023. $2,849,720
International Consultancies
World Bank and International Food Policy Research Institute (2006). Assessment of scientific capacity to support a regional biosafety system in West African Economic and Monetary Union countries. Conducted field visits to Benin and Togo, where I visited research institutes, government agencies, and universities to determine the level of scientific capacity (human and institutional resources) that could be contributed by those countries to a regional biosafety regulatory system.
US Department of Agriculture-Foreign Agricultural Services (2004-2009). Planning and implementation of capacity building activities for the National Biosafety Committee of Serbia-Montenegro. Organized and implemented a series of workshops on biosafety and agricultural biotechnology. Assisted in establishing Institutional Biosafety Committees nationwide.
National Center for Food and Agricultural Policy/US Department of Agriculture (2004). Team member: Served as resource person for technical expertise on agricultural biotechnology during a visit to Thailand. Advised Thai Department of Agriculture scientists on approaches to tracing genetically engineered papaya seeds.
USAID/Agricultural Biotechnology Support Program II/Development Alternatives, Inc (2003). Team member: Conducted an assessment of status of biotechnology research in Indonesia. Made recommendations for USAID activities to strengthen biotechnology capacity in that country, as part of a broader USAID agricultural strategy. Collected technical information to be used in ABSPII priority setting session held August 4-5, 2003.
USAID/Chemonics International., Inc (2003). Conducted an assessment of scientific research project concept notes. Interviewed researchers and made recommendations regarding merit of the projects and requirements for full proposals to be submitted to USAID Uganda.
USAID/Michigan State University (2002-2009). Coordinator of regulatory strategy and product delivery for commercialization of transgenic potato tuber moth potatoes (South Africa).
USAID/Michigan State University (2002-2004). Coordinator of regulatory strategy and product delivery for commercialization of transgenic mustard producing oil enhanced in beta-carotene (India).
Council for Agricultural Science and Technology (2003). Team member: Visited the People’s Republic of China to meet with various regulatory officials as part of a technical exchange project, funded by USDA/FAS.\
USAID/Agricultural Biotechnology Support Program (2002). Team member: Conducted an assessment of status of biotechnology research in South Africa. Made recommendations for USAID activities to strengthen biotechnology capacity in that country (research, commercialization, biosafety regulations, and intellectual property rights).
USAID/Agricultural Biotechnology Support Program (2002). Team Leader: Conducted an assessment of status of biotechnology research in Kenya. Made recommendations for USAID activities to strengthen biotechnology capacity in that country (research, commercialization, biosafety regulations, and intellectual property rights).
USAID/Agricultural Biotechnology Support Program (2002). Team Leader: Conducted an assessment of status of biotechnology research in Uganda. Made recommendations for USAID activities to strengthen biotechnology capacity in that country (research, commercialization, biosafety regulations, and intellectual property rights).
USAID/Development Alternatives,Inc (2001). Developed recommendations for food and feed safety testing guidelines and environmental safety testing guidelines to enable commercialization of genetically engineered crops in Egypt.
USAID (2000). Assisted a multidisciplinary team of consultants in evaluating biotechnology capacity in the Philippines and making recommendations for the allocation of US$30 million PL480 funds earmarked for biotechnology research.
Government of India/Rockefeller Foundation (1998). Evaluated the range of agricultural biotechnology programs in India, identified national needs for testing facilities, made recommendations for the placement and general features of those facilities.
Other
Co-editor, “Policy and Regulation in Bioengineering and Biotechnology “, Frontiers in Bioengineering and Biotechnology 2023. https://www.frontiersin.org/research-topics/45536/policy-and-regulation-in-bioengineering-and-biotechnology
Associate Editor, Frontiers in Bioengineering and Biotechnology, 2022-present.
Member, Core Working Group for “Guidance Framework for Testing Genetically Modified Mosquitoes, Second Edition”, 2021. World Health Organization.
Reviewer, consensus report, “Genetically Engineered Crops: Past Experience and Future Prospects”, 2016. United States National Academies of Sciences Engineering and Medicine.
Member, Core Working Group for “Guidance Framework for Testing of Genetically Modified Mosquitoes”, 2014. World Health Organization.
Science Advisor, International Food Biotechnology Committee, International Life Sciences Institute, 2011-2013.
Preparer, Application for General Release of Transgenic Potato Spunta G2, to South African Executive Committee, July 2008. Collaboration between Michigan State University and the Agricultural Research Council-Vegetable and Ornamental Plant Institute, South Africa.
Associate Editor, Transgenic Research, 2001-present.
Steering Committee Member, Workshop on Research to Improve the Evaluation of the Impact os Genetically Engineered Organisms on Terrestrial and Aquatic Wildlife and Habitats. United States National Research Council, 2007.
Co-organizer, Models of Food Safety Assessment of Transgenic Crops, Workshop funded by USAID and the Rockefeller Foundation, Washington DC, May 6-8, 2003
Co-organizer, Agricultural Biotechnology Short Course, a collaboration between Michigan State University and the USDA/FAS. 2002-2004.]
Member, United States Department of Agriculture Plant Variety Protection Advisory Board, 2002-2004
Author, Petition for exemption from the requirement of a tolerance for Cucurbita pepo L. cultivar YS20 CZW3. Submitted to EPA November 27, 1995.
Co-author, petition for determination of regulatory status of Cucurbita pepo L. cultivar YS20 CZW3. Granted June, 1996.
Author, Petition for exemption from the requirement of a tolerance for Cucurbita pepo L. cultivar YC77E ZW20. Granted November, 1994.
Co-author, petition for determination of regulatory status of Cucurbita pepo L. cultivar YC77E ZW20. Granted December, 1994.
1990-1994: Member, Upjohn Recombinant DNA Advisory Committee; Committee Chairman 1991-1992.
1990-1994: Nonvoting member, Upjohn Biosafety Regulatory Committee.
1991-1992: Secretary, Upjohn Biosafety Regulatory Committee.
Publications
James S, Quemada H, Benedict M, Dass B. 2023. Requirements for market entry of gene drive-modified mosquitoes for control of vector-borne diseases: analogies to other biologic and biotechnology products. Frontiers in Bioengineering and Biotechnology. Vol 11 https://doi.org/10.3389/fbioe.2023.1205865
Taitingfong R, Triplett C, Vásquez V, Rajagopalan R, Raban R, Roberts A, Terradas G, Baumgartner B, Emerson C, Gould F, Okumu F, Schairer C, Bossin H, Buchman L, Campbe K, Clark A, Delborne J, Esvelt K, Fisher J, Friedman R, Gronvall G, Gurfield N,Heitman L, Kofler N, Kuiken T, Kuzma J, Manrique-Saide P, Marshall J,Montague M, Morrison A, Opesen C, Phelan R,Piaggio A, Quemada H, Rudenko L, Sawadogo N, Smith R, Tuten H, Ullah A, Vorsino A, Windbichler N, Akbari O, Long K, Lavery J, Weiss Evans s, Tountas K, Bloss C. 2022. Exploring the value of a global gene drive project registry. Nature Biotechnology https://doi.org/10.1038/s41587-022-01591-w
Tonui W, Ahuja V, Beech C, Connolly J, Dass B, Glandorf D, James S, Muchiri J, Mugoya C, Okoree E, Quemada H, Romeis J. 2022. Points to consider in seeking biosafety approval for research, testing, and environmental release of experimental genetically modified biocontrol products during research and development. Transgenic Research 31: 607–623. https://doi.org/10.1007/s11248-022-00311-z
Quemada, H (2022) Lessons learned from the introduction of genetically engineered crops: relevance to gene drive deployment in Africa. Transgenic Research. https://doi.org/10.1007/s11248-022-00300-2
Devos Y, Mumford JD, Bonsall MB, et al (2021) Risk management recommendations for environmental releases of gene drive modified insects. Biotechnology Advances 107807. https://doi.org/10.1016/j.biotechadv.2021.107807
Entine J, Felipe MSS, Groenewald J-H, et al (2021) Regulatory approaches for genome edited agricultural plants in select countries and jurisdictions around the world. Transgenic Research. 10.1007/s11248-021-00257-8
Mbabazi R, Quemada H, Shore S, et al (2020) Regulatory Capacity Strengthening for Gene-Drive Technology Applications for Vector-Borne Disease Control in Africa. Michigan State University. WorldTAP Policy Brief 7
Kausch, A.P, Nelson-Vasilchik, K., Hague, J., Mookkan, M., Quemada, H., Dellaporta, S., Fragoso, C., Zhang, A. 2019. Edit at Will: Genotype Independent Plant Transformation in the Era of Advanced Genomics and Genome Editing. Plant Science. doi: 10.1016/j.plantsci.2019.01.006
James, S., Collins, F., Welkhoff, P., Emerson, C., Godfray, H., Gottlieb, M., Greenwood, B., Lindsay, S., Mbogo, C., Okumu, F., Quemada, H., Savadogo, M., Singh, J., Tountas, K., Touré, Y. 2018. Pathway to Deployment of Gene Drive Mosquitoes as a Potential Biocontrol Tool for Elimination of Malaria in Sub-Saharan Africa: Recommendations of a Scientific Working Group. The American Journal of Tropical Medicine and Hygiene 98: 1-49.
Adenle, A., Morris, E., Murphy, D., Phillips, P., Trigo, E., Kearns, P., Li, Y-H., Quemada, H., Falck-Zepeda, J., Komen, J. 2018. Rationalizing governance of genetically modified products in developing countries. Nature Biotechnology 36: 137-139.
Lemgo, G., Nehra, N., Quemada, H. Food safety assessment of genetically modified crops in developing countries: the experience in Africa. In “Genetically Modified Organisms in Developing Countries: Risk Analysis and Governance.” A. Adenle, E. Morris, D. Murphy, eds. Cambridge University Press, Cambridge. pp. 103-114.
Adelman, Z., Akbari, O., Bauer, J., Bier, E., Bloss, C., Carter, S., Callender, C., Costero-Saint Denis, A., Cowhey, P., Dass, B., Delborne, J., Devereaux, M., Ellsworth, P., Friedman, R., Gantz, V., Gibson, C., Hay. B., Hoddle, M., James, A., James, S., Jorgenson, L., Kalichman, M., Marshall, J., McGinnis, W., Newman, J., Pearson, A., Quemada, H., Rudenko, L., Shelton, A., Vinetz, J., Weisman, J., Wong, B., & Wozniak, C. 2017. Rules of the road for insect gene drive research and testing. Nature Biotechnology 35: 716-718.
Roberts, A., de Andrade, P.P., Okumu, F., Quemada, H., Savadogo, M., Singh, J.A. and James, S. 2017. Results from the Workshop “Problem Formulation for the Use of Gene Drive in Mosquitoes”. The American Journal of Tropical Medicine and Hygiene 96: 530-533.
Quemada, H. 2016. Regulation of Transgenic Mosquitoes. In “Genetic Control of Malaria and Dengue.” Z. Adelman, ed. Elsevier, London. pp. 363-373. Published online November 28, 2016, doi: 10.4269/ajtmh.16-0726.
Haggman, H., A. Raybould, A. Borem, T. Fox, L. Handley, M. Hertzberg, M. Lu, P. Macdonald, T. Oguchi, G. Pasquali, L. Pearson, G. Peter, H. Quemada, A.Seguin, K. Tattersall, E. Ulian, C. Walter and M. McLean. 2013. Genetically engineered trees for plantation forests: key considerations for environmental risk assessment. Plant Biotechnology Journal doi: 10.1111/pbi.12100.
Henley, W., R.W. Litaker, L. Novoveská, C. Duke, H. Quemada, and R. Sayre. 2013. Initial risk assessment of genetically modified (GM) microalgae for commodity-scale biofuel cultivation. Algal Research 2: 66–77.
Hancock, J., and Quemada, H. 2011. A problem-based approach to environmental risk assessment of genetically engineered crops. In Grumet, Rebecca & Hancock, F. & Maredia, M. & Weebadde, Cholani. Environmental Safety of Genetically Engineered Crops. East Lansing: Michigan State University Press. Project MUSE.
Zarka, K., R. Greyling, I. Gazendam, D. Oloefse , K. Felcher, G. Bothma, J. Brink, H. Quemada, and D. Douches. 2010. Insertion and Characterization of the cry1Ia1 Gene in the Potato Cultivar Spunta for Resistance to Potato Tuber Moth. Journal of the American Society for Horticultural Science 135: 317–324.
Quemada, H, K. Zarka, W. Pett, G. Bothma, K. Felcher, H. Mirendil, M. Koch, J. Brink and D. Douches. 2010. Safety Evaluations of the Cry1Ia1 Protein Found in the Transgenic Potato ‘SpuntaG2’. Journal of the American Society for Horticultural Science 135: 325–332.
Douches, D., W. Pett, D. Visser, J. Coombs, K, Zarka, K. Felcher, G. Bothma, J. Brink, M. Koch, and H. Quemada. 2010. Field and Storage Evaluations of ‘SpuntaG2’ for Resistance to Potato Tuber Moth and Agronomic Performance. Journal of the American Society for Horticultural Science 135: 333–340.
Raybould, A. and H. Quemada. 2010. Bt crops and food security in developing countries: realised benefits, sustainable use and lowering barriers to adoption. Food Security 2: 247–259.
Ramessar, K., T. Capell, R.M. Twyman, H. Quemada, and P. Christou. 2009. Calling the tunes on transgenic crops: the case for regulatory harmony. Molecular Breeding 23: 99–112.
Sengooba, T., R. Grumet, J. Hancock, B. Zawedde, L. Kitandu, C. Weebadde, M. Karembu, E. Kenya, K. Maredia, P. Nampala, J. Ochanda, H. Quemada, M. Rubindamayugi. 2009. Biosafety education relevant to genetically engineered crops for academic and non-academic stakeholders in East Africa. Electronic Journal of Biotechnology 12: 1-5.
Douches, D., J. Brink, H. Quemada, W. Pett, M. Koch, D. Visser, K, Maredia and K. Zarka. 2008. Commercialization of potato tuber moth tesistant potatoes in South Africa. In Kroschel J and L Lacey (eds.), “Integrated Pest Management for the Potato Tuber Moth, Phthorimaea operculella Zeller – a Potato Pest of Global Importance. Tropical Agriculture 20, Advances in Crop Research 10. Margraf Publishers, Weikersheim, Germany, 139-147.
Quemada, H., L. Strehlow, D. Decker-Walters, and J.E. Staub. 2008. Population Size and Incidence of Virus Infection in Free-Living Populations of Cucurbita pepo. Environmental Biosafety Research, 7: 185-196.
Ramessar, K., T. Capell, R.M. Twyman, H. Quemada, and P. Christou. 2008. Calling the tunes on transgenic crops: the case for regulatory harmony. Molecular Breeding DOI 10.1007/s11032-008-9217-z.
Ramessar K., T. Capell, R.M. Twyman, H. Quemada and P. Christou. 2008. Trace and Traceability–A Call for Regulatory Harmony. Nature Biotechnology 26: 975 - 978.
Quemada, Hector D., David M. Tricoli, Jack E. Staub, Eileen A. Kabelka, Yi-Hong Wang. 2008. Cucurbits. In "Compendium of Transgenic Crop Plants: Transgenic Vegetable Crops". C. Kole and T.C. Hall, eds. Blackwell Publishing, Oxford, UK, pp 145-184.
Matten, S., G.P. Head, and H.D. Quemada. 2008. How governmental regulation can help or hinder the integration of Bt crops within IPM programs. In Integration of Insect-Resistant Genetically Modified Crops Within IPM Programs (J. Romeis, AM Shelton, and GG Kennedy, eds). Springer. pp. 27-39.
Romeis, J., Bartsch, D., Bigler, F., Candolfi, M.P., Gielkens, M.P.C., Hartley, S., Hellmich, R.L., Huesing, J.E., Jepson, P.C., Layton, R., Quemada, H., Raybould, A., Rose, R.I., Schiemann, J., Sears, M.K., Shelton, A.M., Sweet, J., Vaituzis, Z., Wolt, J.D. 2008. Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nature Biotechnology 26: 203-208.
Linacre, N.A., J. Gaskell, M. W. Rosegrant, J. Falck-Zepeda, H. Quemada, M. Halsey and R. Birner. 2006. Strategic environmental assessments for genetically modified organisms. Impact Assessment and Project Appraisal 24: 35-43.
Linacre, N.A.; Gaskell, J., Rosegrant, M.W.; Falck-Zepeda, J.; Quemada, H.; Halsey, M.; Birner, R. 2005. Analysis for Biotechnology Innovations Using Strategic Environmental Assessment (SEA). International Food Policy Research Institute: Washington, D.C.
Atanassov, A.; Bahieldin, A.; Brink, J.; Burachik, M.; Cohen, J.I.; Dhawan, V.; Ebora, R.V.; Falck-Zepeda, J.; Herrera-Estrella, L.; Komen, J.; Low, F.C.; Omaliko, E.; Odhiambo, B.; Quemada, H.; Peng, Y.: Sampaio, M.J.; Sithole-Niang, I.; Sittenfeld, A.; Smale, M.; Sutrisno, Valyasevi, R.; Zafar, Y. Zambrano, P. 2004. To Reach the Poor: Results from the ISNAR-IFPRI Next Harvest Study on Genetically Modified Crops, Public Research, and Policy Implications. EPTD Discussion Paper No. 116. Washington, D.C., International Food Policy Research Institute.
Cohen, J.I., Quemada, H., Frederick, R. 2003. Food Safety and GM Crops: Implications for Developing-Country Research. In Food Safety in Food Security and Food Trade. Focus 10, Brief 16 of 17. Washington, D.C., International Food Policy Research Institute.
Decker-Walters, D.S.; Chung., S.; Staub, J.E.; Quemada, H.D.; Lopez-Sese, A.I. 2002. The origin and genetic affinities of wild populations of melon (Cucumis melo, Cucurbitaceae) in North America. Plant Systematics and Evolution 233:183-197.
Quemada, H. 2002. Case Study: Virus resistant crops. In GeneticallyModified Crops: Assessing Safety. (Atherton, K., ed..). Taylor & Francis : New York and London. pp. 219-240.
Decker-Walters, D. S., J. E. Staub, S. M. Chung, E. Nakata, and H. D. Quemada. 2001. Diversity in free-living populations of Cucurbita pepo as assessed by random amplified polymorphic DNA. Systematic Botany 27:19-28.
Pang, S.; Jan, F.; Tricoli, D. M.; Russell, P. F.; Carney, K. J.; Hu, J. S.; Fuchs, M.; Quemada, H. D.; Gonsalves, D. 2000. Resistance to squash mosaic comovirus in transgenic squash plants expressing its coat protein genes. Molecular Breeding 6:87-93.
Fuchs, M., J.R. McFerson, D.M. Tricoli, J.R. McMaster, RZ. Deng, M.L. Boeshore, J.F. Reynolds, P.F. Russell, H.D. Quemada, and D. Gonsalves, 1997. Cantaloupe line CZW-30 containing coat protein genes of cucumber mosaic virus, zucchini yellow mosaic virus, and watermelon mosaic virus-2 is resistant to these aphid-borne viruses in the field. Molecular Breeding 3: 279-290.
Pang, S-Z, F-J Jan, D.M. Tricoli, H.D. Quemada, and D. Gonsalves, 1996. Post-transcriptional transgene silencing and consequent tospovirus resistance in transgenic lettuce are affected by transgene dosage, plant development, and environmental factors. The Plant Journal 9: 899-909.
Tricoli, D.M., K.J. Carney, P.F. Russell, J.R. McMaster, D.W. Groff, K.C. Hadden, P.T. Himmel, J.P. Hubbard, M.L. Boeshore, and H.D. Quemada, 1995. Field Evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to cucumber mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. BioTechnology 13: 1458-1465.
Quemada, H.D., 1994. The Asgrow Seed Company’s experience with vegetable biotechnology. In Biosafety for Sustainable Agriculture: Sharing Biotechnology Regulatory Experiences of the Western Hemisphere (Krattiger, A.F., and A. Rosemarin, eds.). ISAAA: Ithaca & SEI: Stockholm. pp. 167-173.
Quemada, H., D. Gonsalves, and J.L. Slightom, 1991. CMV-C coat protein gene expression in tobacco: protection against infection by CMV strains transmitted mechanically or by aphids. Phytopathology 81: 794-802.
Quemada, H., L.C. Sieu, D.R. Siemieniak, D. Gonsalves, and J.L. Slightom, 1990. Watermelon mosaic virus II and zucchini yellow mosaic virus: cloning of 3’-terminal regions, nucleotide sequences, and phylogenetic comparisons. Journal of General Virology 71: 1451-1460.
Quemada, H., B. L’Hostis, D. Gonsalves, I. Reardon, R. Heinrikson, E.L. Hiebert, L.C. Sieu, and J. L. Slightom, 1990. The nucleotide sequences of cDNA clones of the 3’ terminal regions of papaya ringspot virus strains W and P. Journal of General Virology 71: 203-210.
Quemada, H., C. Kearney, D. Gonsalves, and J.L. Slightom, 1989. Nucleotide sequences of the coat protein genes and flanking regions of cucumber mosaic virus strains C and WL RNA 3. Journal of General Virology 70: 1065-1073.
Slightom, J.L. and H.D. Quemada, 1988. Procedures for constructing ds-cDNA clone banks. In: Plant Molecular Biology (eds. S.B. Gelvin and R.A. Schilperoort), Martinus Nijhoff, Dordecht, The Netherlands.
Quemada, H., E.J. Roth, and K.G. Lark, 1987. Changes in methylation of tissue cultured soybean cells detected by digestion with the restriction enzymes HpaII and MspI. Plant Cell Reports 6: 63-66.
Patents
U.S.: #6337431 “Transgenic plants expressing DNA constructs containing a plurality of genes to impart virus resistance.”
#5,349,128 “Cucumber Mosaic Virus Coat Protein Gene.”
#5,623,066 “Cucumber Mosaic Virus Coat Protein Gene.”.
Canada: #1,335,965 “Cucumber Mosaic Virus Coat Protein Gene”.
Australia: #621336 “Cucumber Mosaic Virus Coat Protein Gene”.
#634168 “Potyvirus Coat Protein Genes and Plants Transformed Therewith”.
#634171 “Cucumber Mosaic Virus Coat Protein Gene”.
#639891 “Expression Cassette for Plants”.
Europe: E 107361 (Austria). “CMV coat protein gene.”
Notices of allowance received on others.